Travellers will be able to enter Queensland by providing a negative rapid antigen test from January 1, Annastascia Palaszczuk has announced.
It follows pressure from other states to drop a rule requiring people from hotspots such as Victoria and NSW to take a PCR Covid test 72-hours before departure.
"From January 1 travellers into Qld from interstate hotspots can use a negative Rapid Antigen Test to satisfy border pass requirements," Premier Annastascia Palaszczuk tweeted on Wednesday morning.
"A PCR test will no longer be required."
The groundbreaking change comes as the state records another uptick in COVID-19 cases, reporting 1589 new cases in the past 24 hours.
There are 6368 active cases, but no patients are being treated in intensive care.
Omicron continues to be the more dominant strain of the virus, with almost 80 per cent of cases identified as the new variant.
Authorities said 35,002 tests were conducted, with another warning that numbers will continue to rise.
Chief Health Officer John Gerrard said the move to rapid antigen testing was appropriate.
"I advised the premier that rapid antigen testing was an appropriate form of testing in the current environment for interstate travellers from hotspots like New South Wales," Dr Gerrard said.
He deflected concerns that the supply and availability of the test was an issue.
"Rapid antigen tests in Queensland at the moment are difficult to obtain ... we are led to believe they are available in New South Wales," Dr Gerrard said.
"The value of rapid antigen tests, as I'm sure most Australians are now aware, is in the context of widescale transmission of the virus.
"They are not as sensitive as PCR, so they can miss a proportion of positive cases, but in the context of widescale transmission, that's where they start to become useful - and we're entering that phase now."
Travellers will have to declare the results of their RAT in border applications.
Queensland's tough testing regime for visitors has come under fire, particularly from NSW.
NSW Premier Dominic Perrottet begged the Queensland government to ease border entry requirements,.
Visitors to Queensland had required a negative PCR test within 72 hours of coming to the state.
Mr Perrottet said the "tourism testing" had clogged an overwhelmed testing system.
Thousands of travellers only added to massive testing queues, waiting for hours to be swabbed.
Wait times for results are even longer, with the usual 24-hour turnaround blowing out to five days in some cases.
"There are people getting tests who don't have any symptoms, are not feeling unwell ... and are taking the place of people who are unwell or who are required to get a test by NSW Health," Mr Perrottet said.
Queensland's RAT change comes a day after completely scrapping the controversial day five PCR tests which authorities conceded wasn't "contributing in any way" to keeping the community safe.
Of the tens of thousands of travellers who have crossed state lines since the Queensland border reopened, only 0.6 per cent have recorded a positive result in the day five test.
with Australian Associated Press